1. 产品参数:ex(nm): 562 em(nm):576分 子 量:1015.79 溶剂: dmso存储条件 :在零下15度以下保存,避免光照
2. 产品概述:
钙黄绿素红,am是美国aat bioquest生产的用于检测活细胞的荧光探针,calcein am是用于标记和检测活细胞细胞功能的流行的荧光探针之一。然而,calcein am的单色使得在多色应用中不可能使用这种有价值的试剂。例如,由于与gfp相同的颜色,不可能将calcein am与gfp-转化细胞组合使用。为了解决calcein am的这种颜色限制,我们开发了calcein orange ,calcein red 和calcein deep red 。这些新的calcein am类似物与calcein am结合使用,可以对活细胞进行多色标记和功能分析。非荧光calcein red am可以轻松进入活细胞并水解产生强荧光calcein red 染料。可以使用常见的tritc / cy3滤波片组检测calcein red 染料。百萤生物是aat bioquest的中国代理商,为您提供优质的钙黄绿素红,am。
3. 适用仪器:(1)流式细胞仪
ex: 532/561nm
em:585/40nm
(2)荧光酶标仪
ex: 540nm
em: 590nm
cutoff: 570nm
推荐孔板: 黑色透明底板
读取模式: 底读模式
(3)荧光显微镜
ex: tritc滤波片组
em: tritc滤波片组
推荐孔板: 黑色透明底板
4. 参考文献a perfused human blood--brain barrier on-a-chip for high-throughput assessment of barrier function and antibody transport
authors: nienke r wevers, dhanesh g kasi, taylor gray, karlijn j wilschut, benjamin smith, remko van vught, fumitaka shimizu, yasuteru sano, takashi kanda, graham marsh
journal: fluids and barriers of the cns (2018): 23
astragaloside iv protects blood-brain barrier integrity from lps-induced disruption via activating nrf2 antioxidant signaling pathway in mice
authors: hongli li, ping wang, fei huang, jinmei jin, hui wu, beibei zhang, zhifei wang, hailian shi, xiaojun wu
journal: toxicology and applied pharmacology (2017)
functional imaging of neuronal activity of auditory cortex by using cal-520 in anesthetized and awake mice
authors: jingcheng li, jianxiong zhang, meng wang, junxia pan, xiaowei chen, xiang liao
journal: biomedical optics express (2017): 2599--2610
ninj2--a novel regulator of endothelial inflammation and activation
authors: jingjing wang, jingjing fa, pengyun wang, xinzhen jia, huixin peng, jing chen, yifan wang, chenhui wang, qiuyun chen, xin tu
journal: cellular signalling (2017)
erythropoietin stimulates endothelial progenitor cells to induce endothelialization in an aneurysm neck after coil embolization by modulating vascular endothelial growth factor
authors: peixi liu, yingjie zhou, qingzhu an, yaying song, xi chen, guo-yuan yang, wei zhu
journal: medicine (2016): 1--8
flexible endoscopic spray application of respiratory epithelial cells as platform technology to apply cells in tubular organs
authors: anja lena thiebes, manuel armin reddemann, johannes palmer, reinhold kneer, stefan jockenhoevel, christian gabriel cornelissen
journal: tissue engineering part c: methods (2016): 322--331
influence of hypothermia and subsequent rewarming upon leukocyte-endothelial interactions and expression of junctional-adhesion-molecules a and b
authors: nicolai v bogert, isabella werner, angela kornberger, patrick meybohm, anton moritz, till keller, ulrich a stock, andres beiras-fernandez
journal: scientific reports (2016)
inhibition of abc transport proteins by oil sands process affected water
authors: hattan a alharbi, david mv saunders, ahmed al-mousa, jane alcorn, alberto s pereira, jonathan w martin, john p giesy, steve b wiseman
journal: aquatic toxicology (2016): 81--88
rapid generation of collagen-based microtissues to study cell--matrix interactions
authors: marie-elena brett, alexandra l crampton, david k wood
journal: technology (2016): 1--8
toxicokinetics and toxicodynamics of chlorpyrifos is altered in embryos of japanese medaka exposed to oil sands process-affected water: evidence for inhibition of p-glycoprotein
authors: hattan a alharbi, jane alcorn, ahmed al-mousa, john p giesy, steve b wiseman
journal: journal of applied toxicology (2016)
关键词:荧光显微镜 荧光酶标仪 流式细胞仪
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