热休克蛋白β2（HSPβ2）检测试剂盒说明书
热休克蛋白β2(hspβ2)检测试剂盒
适用生物 homo sapiens (human，人)
检测范围 0.625-40ng/ml 灵敏度 0.244ng/ml
样本类型 serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96t
热休克蛋白β2(hspβ2)检测试剂盒
elisa kit for heat shock protein beta 2 (hspb2)
for in vitro and research use only, not for use in clinical diagnostic procedures!
organism species homo sapiens (human)
product no. sea871hu
sample type serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
format 96-well strip plate
assay length 4.5 hours
detection range 0.625-40ng/ml the standard curve concentrations used for the elisa’s were 40ng/ml, 20ng/ml, 10ng/ml, 5ng/ml, 2.5ng/ml, 1.25ng/ml, 0.625ng/ml
sensitivity the minimum detectable dose of this kit is typically less than 0.244ng/ml.
specificity
this assay has high sensitivity and excellent specificity for detection of heat shock protein beta 2 (hspb2).
no significant cross-reactivity or interference between heat shock protein beta 2 (hspb2) and analogues was observed.
recovery
matrices listed below were spiked with certain level of recombinant heat shock protein beta 2 (hspb2) and the recovery rates were calculated by comparing the measured value to the expected amount of heat shock protein beta 2 (hspb2) in samples.
热休克蛋白β2(hspβ2)检测试剂盒
matrix recovery range (%) average(%)
serum(n=5) 91-105 101
edta plasma(n=5) 78-97 93
heparin plasma(n=5) 89-99 94
precision
intra-assay precision (precision within an assay): 3 samples with low, middle and high level heat shock protein beta 2 (hspb2) were tested 20 times on one plate, respectively.
inter-assay precision (precision between assays): 3 samples with low, middle and high level heat shock protein beta 2 (hspb2) were tested on 3 different plates, 8 replicates in each plate.
cv(%) = sd/meanx100
intra-assay: cv<10%
inter-assay: cv<12%
linearity
the linearity of the kit was assayed by testing samples spiked with appropriate concentration of heat shock protein beta 2 (hspb2) and their serial dilutions. the results were demonstrated by the percentage of calculated concentration to the expected.
sample 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 97-104% 80-104% 78-104%
edta plasma(n=5) 92-101% 87-98% 95-102% 93-101%
heparin plasma(n=5) 82-102% 83-99% 82-96% 91-105%
stability
the stability of kit is determined by the loss rate of activity. the loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
to minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. it is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
reagents and materials provided
reagents quantity reagents quantity
pre-coated, ready to use 96-well strip plate 1 plate sealer for 96 wells 4
standard 2 standard diluent 1×20ml
detection reagent a 1×120μl assay diluent a 1×12ml
detection reagent b 1×120μl assay diluent b 1×12ml
tmb substrate 1×9ml stop solution 1×6ml
wash buffer (30 × concentrate) 1×20ml instruction manual 1
assay procedure summary
1. prepare all reagents, samples and standards;
2. add 100μl standard or sample to each well. incubate 2 hours at 37oc;
3. aspirate and add 100μl prepared detection reagent a. incubate 1 hour at 37oc;
4. aspirate and wash 3 times;
5. add 100μl prepared detection reagent b. incubate 30 minutes at 37oc;
6. aspirate and wash 5 times;
7. add 90μl substrate solution. incubate 15-25 minutes at 37oc;
8. add 50μl stop solution. read at 450nm immediay.
test principle
the test principle applied in this kit is sandwich enzyme immunoassay. the microtiter plate provided in this kit has been pre-coated with an antibody specific to heat shock protein beta 2 (hspb2). standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to heat shock protein beta 2 (hspb2). next, avidin conjugated to horseradish peroxidase (hrp) is added to each microplate well and incubated. after tmb substrate solution is added, only those wells that contain heat shock protein beta 2 (hspb2), biotin-conjugated antibody and enzyme-conjugated avidin will exhibit a change in color. the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. the concentration of heat shock protein beta 2 (hspb2) in the samples is then determined by comparing the o.d. of the samples to the standard curve.
高效反渗透水处理系统
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热休克蛋白β2（HSPβ2）检测试剂盒说明书
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对于冷凝器胶球清洗装置的优化设计，江苏沛德有一套！
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低温等离子处理机对氧化锆粘接能力可以提升20倍强度
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2000赫兹水管漏水测漏仪生产厂家
适用生物 homo sapiens (human，人)
检测范围 0.625-40ng/ml 灵敏度 0.244ng/ml
样本类型 serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96t
热休克蛋白β2(hspβ2)检测试剂盒
elisa kit for heat shock protein beta 2 (hspb2)
for in vitro and research use only, not for use in clinical diagnostic procedures!
organism species homo sapiens (human)
product no. sea871hu
sample type serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
format 96-well strip plate
assay length 4.5 hours
detection range 0.625-40ng/ml the standard curve concentrations used for the elisa’s were 40ng/ml, 20ng/ml, 10ng/ml, 5ng/ml, 2.5ng/ml, 1.25ng/ml, 0.625ng/ml
sensitivity the minimum detectable dose of this kit is typically less than 0.244ng/ml.
specificity
this assay has high sensitivity and excellent specificity for detection of heat shock protein beta 2 (hspb2).
no significant cross-reactivity or interference between heat shock protein beta 2 (hspb2) and analogues was observed.
recovery
matrices listed below were spiked with certain level of recombinant heat shock protein beta 2 (hspb2) and the recovery rates were calculated by comparing the measured value to the expected amount of heat shock protein beta 2 (hspb2) in samples.
热休克蛋白β2(hspβ2)检测试剂盒
matrix recovery range (%) average(%)
serum(n=5) 91-105 101
edta plasma(n=5) 78-97 93
heparin plasma(n=5) 89-99 94
precision
intra-assay precision (precision within an assay): 3 samples with low, middle and high level heat shock protein beta 2 (hspb2) were tested 20 times on one plate, respectively.
inter-assay precision (precision between assays): 3 samples with low, middle and high level heat shock protein beta 2 (hspb2) were tested on 3 different plates, 8 replicates in each plate.
cv(%) = sd/meanx100
intra-assay: cv<10%
inter-assay: cv<12%
linearity
the linearity of the kit was assayed by testing samples spiked with appropriate concentration of heat shock protein beta 2 (hspb2) and their serial dilutions. the results were demonstrated by the percentage of calculated concentration to the expected.
sample 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 97-104% 80-104% 78-104%
edta plasma(n=5) 92-101% 87-98% 95-102% 93-101%
heparin plasma(n=5) 82-102% 83-99% 82-96% 91-105%
stability
the stability of kit is determined by the loss rate of activity. the loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
to minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. it is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
reagents and materials provided
reagents quantity reagents quantity
pre-coated, ready to use 96-well strip plate 1 plate sealer for 96 wells 4
standard 2 standard diluent 1×20ml
detection reagent a 1×120μl assay diluent a 1×12ml
detection reagent b 1×120μl assay diluent b 1×12ml
tmb substrate 1×9ml stop solution 1×6ml
wash buffer (30 × concentrate) 1×20ml instruction manual 1
assay procedure summary
1. prepare all reagents, samples and standards;
2. add 100μl standard or sample to each well. incubate 2 hours at 37oc;
3. aspirate and add 100μl prepared detection reagent a. incubate 1 hour at 37oc;
4. aspirate and wash 3 times;
5. add 100μl prepared detection reagent b. incubate 30 minutes at 37oc;
6. aspirate and wash 5 times;
7. add 90μl substrate solution. incubate 15-25 minutes at 37oc;
8. add 50μl stop solution. read at 450nm immediay.
test principle
the test principle applied in this kit is sandwich enzyme immunoassay. the microtiter plate provided in this kit has been pre-coated with an antibody specific to heat shock protein beta 2 (hspb2). standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to heat shock protein beta 2 (hspb2). next, avidin conjugated to horseradish peroxidase (hrp) is added to each microplate well and incubated. after tmb substrate solution is added, only those wells that contain heat shock protein beta 2 (hspb2), biotin-conjugated antibody and enzyme-conjugated avidin will exhibit a change in color. the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. the concentration of heat shock protein beta 2 (hspb2) in the samples is then determined by comparing the o.d. of the samples to the standard curve.
高效反渗透水处理系统
数控工具磨床的安全操作事项
恒电位仪的使用注意事项
NACHI不二越电磁阀使用性能及特点
四丁基六氟磷酸铵可极大程度地降低体系的电阻率
热休克蛋白β2（HSPβ2）检测试剂盒说明书
这七种塑胶跑道检测的标准与方法！快来了解下吧
电缆防火涂料*的价格
毛刷清洗机结构特点及性能优势
对于冷凝器胶球清洗装置的优化设计，江苏沛德有一套！
实验动物安乐死与福利伦理
第三方水质检测机构是保障饮用水安全的重要角色
加长杆衬氟蝶阀D341F46蜗轮加长杆衬氟法兰蝶阀的特点
使用光储充系统能实现能源的有效利用
低温等离子处理机对氧化锆粘接能力可以提升20倍强度
小鼠Smad7 elisa检测试剂盒操作步骤
便携式气味传感器 POLFA的优势功能介绍
柱后衍生的简单介绍
JB-300B摆锤式冲击试验机、300j低价实惠冲击试验机
2000赫兹水管漏水测漏仪生产厂家