海肾荧光素酶测定试剂盒2.0是biotium的海肾荧光素酶测定试剂盒(目录号30004)的改进版本,具有简化的方案和改进的工作溶液稳定性。该试剂盒还具有biotium的水溶性aquaphile腔肠素。
艾美捷海肾荧光素酶测定试剂盒2.0,150次测定#btm-30082-1组件:
5x 被动裂解缓冲液
海肾萤光素酶检测缓冲液2.0
aquaphile™ 腔肠素
海肾荧光素酶测定试剂盒相关特征:
高灵敏度和线性快速型rluc 测定
易于处理的水溶性基材
高性价比
海肾荧光素酶测定试剂盒属性:
化验类型/选项:终点测定,发光(闪光型)
检测方法/读数:光度计(带试剂注射器的单管或微量板读取器)
储存条件:在-60°c或更低温度下储存
sea kidney luciferase has been used as a reporter gene to study gene regulation and function in vitro and in vivo. it is usually used for multiple transcriptional reporter gene detection or as a standardized transfection control for firefly luciferase detection. renila luciferase is a 36000 dalton monomer protein that catalyzes the oxidation of coelenterazine by oxygen to produce light. the activity of this enzyme does not require post translational modification and can serve as a genetic reporter gene immediately after translation. coelenterazine also emits light through enzyme independent oxidation, a process called self luminescence. superoxide anions and peroxynitrite in cells and tissues enhance self luminescence. this test kit uses a special buffer formula, which aims to conduct reliable linear measurement of luciferase activity in the sea and kidney with the minimum self luminous background and excellent sensitivity.
note: this reagent kit is a flash type luminescence detection that requires immediate measurement of luminescence after adding reagents to your sample. it requires a single tube luminescent meter or a luminescent enzyme-linked immunosorbent assay instrument with a reagent syringe.
easy to use for consistent results
this kit is a new improved substitute for haishen luciferase test kit (catalog no. 30004). in addition to providing a simplified experimental scheme, renilla luciferase test kit 2.0 also has biotium aquaphile ™ coelenterazine, a water-soluble substrate, is different from coelenterazine's methanol solution. it can be stored at - 20 ° c with the minimum evaporation.
the measurement quantity is based on a 96 well measurement format. this kit contains sufficient passive lysis buffer to perform the specified number of measurements within the plate size range of 96 to 24 wells.
导热油冷热循环一体机非标定制
无线网桥建立点对多点传输的注意事项快马科技
接地导通测试仪介绍及使用注意事项
百超激光切割机的稳压电源有什么用途?
贵州遵义红花岗水利大口径TPEP防腐钢管生产
解决方案丨艾美捷海肾荧光素酶测定试剂盒
防火泥封堵施工标准
密封性测试仪的原理与应用
畜禽养殖废气处理的应用领域广泛,主要包括以下方面
博迅真空干燥箱的维护保养方法
MAY-2001 助焊剂浓度比重在线监测仪 稀释剂自动调配比例
调节阀使用维护和保养
知道吗,高压清洗机维修要定期进行
“污”尽其用,废水如何资源化
德国BURKERT宝德电磁阀的阀门压力试验初探测试
力士乐 叠加阀板 Z4S 16
LC483/5nm ML1240/620mm海德汉光栅尺德国技术
玻璃反应釜怎样延长使用寿命
五金件冲压、五金冲压件加工特点和优势
猪滑液支原体探针法荧光定量PCR检测试剂盒准备试剂与收集血样