90plus 纳米粒度仪应用案例-14
90plus纳米粒度仪应用案例
文献名：surfactant–polymernanoparticlesovercomep-glycoprotein-mediateddrugefflux
作者：maheshd.chavanpatil,aymankhdair,brigittegerard,corbinbachmeier,donaldw.miller,malathyp.v.shekharandjayanthpanyam
departmentofpharmaceuticalsciences,eugeneapplebaumcollegeofpharmacyandhealthsciences,waynestateuniversity,detroit,michigan48201,breastcancerprogram,karmanoscancerinstitute,110eastwarrenavenue,detroit,michigan48201,departmentofpharmaceuticalsciences,collegeofpharmacy,universityofnebraskamedicalcenter,omaha,nebraska68198-6025,departmentofpharmacologyandtherapeutics,universityofmanitoba,winnipeg,mb,r3e0t6canada,anddepartmentofpathology,waynestateuniversityschoolofmedicine,detroit,michigan48201
摘要：nanoparticlesenhancethetherapeuticefficacyofanencapsulateddrugbyincreasingandsustainingthedeliveryofthedruginsidethecell.wehavepreviouslydemonstratedthataerosolot(aot)–alginatenanoparticles,anovelformulationdevelopedrecentlyinourlaboratory,significantlyenhancethetherapeuticefficacyofencapsulateddrugslikedoxorubicinindrug-sensitivetumorcells.thepurposeofthisstudyistoevaluatethedrugdeliverypotentialofaot–alginatenanoparticlesindrug-resistantcellsoverexpressingthedrugeffluxtransporter,p-glycoprotein(p-gp).aot–alginatenanoparticleswereformulatedusinganemulsion–cross-linkingprocess.rhodamine123anddoxorubicinwereusedasmodelp-gpsubstrates.cytotoxicityofnanoparticle-encapsulateddoxorubicinandkineticsofnanoparticle-mediatedcellulardrugdeliverywereevaluatedinbothdrug-sensitiveand-resistantcelllines.aot–alginatenanoparticlesenhancedthecytotoxicityofdoxorubicinsignificantlyindrug-resistantcells.theenhancementincytotoxicitywithnanoparticleswassustainedoveraperiodof10days.uptakestudieswithrhodamine-loadednanoparticlesindicatedthatnanoparticlessignificantlyincreasedthelevelofdrugaccumulationinresistantcellsatnanoparticledoseshigherthan200µg/ml.blanknanoparticlesalsoimprovedrhodamineaccumulationindrug-resistantcellsinadose-dependentmanner.nanoparticle-mediatedenhancementinrhodamineaccumulationwasnotbecauseofmembranepermeabilization.fluorescencemicroscopystudiesdemonstratedthatnanoparticle-encapsulateddoxorubicinwaspredominantlylocalizedintheperinuclearvesiclesandtoalesserextentinthenucleus,whereasfreedoxorubicinaccumulatedmainlyinperipheralendocyticvesicles.inhibitionofp-gp-mediatedrhodamineeffluxwithaot–alginatenanoparticleswasconfirmedinprimarybrainmicrovesselendothelialcells.inconclusion,anaot–alginatenanoparticlesystemenhancedthecellulardeliveryandtherapeuticefficacyofp-gpsubstratesinp-gp-overexpressingcells.
台式循环水真空泵使用方法
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2018年聚氨酯保温板价格及报价
90plus 纳米粒度仪应用案例-14
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文献名：surfactant–polymernanoparticlesovercomep-glycoprotein-mediateddrugefflux
作者：maheshd.chavanpatil,aymankhdair,brigittegerard,corbinbachmeier,donaldw.miller,malathyp.v.shekharandjayanthpanyam
departmentofpharmaceuticalsciences,eugeneapplebaumcollegeofpharmacyandhealthsciences,waynestateuniversity,detroit,michigan48201,breastcancerprogram,karmanoscancerinstitute,110eastwarrenavenue,detroit,michigan48201,departmentofpharmaceuticalsciences,collegeofpharmacy,universityofnebraskamedicalcenter,omaha,nebraska68198-6025,departmentofpharmacologyandtherapeutics,universityofmanitoba,winnipeg,mb,r3e0t6canada,anddepartmentofpathology,waynestateuniversityschoolofmedicine,detroit,michigan48201
摘要：nanoparticlesenhancethetherapeuticefficacyofanencapsulateddrugbyincreasingandsustainingthedeliveryofthedruginsidethecell.wehavepreviouslydemonstratedthataerosolot(aot)–alginatenanoparticles,anovelformulationdevelopedrecentlyinourlaboratory,significantlyenhancethetherapeuticefficacyofencapsulateddrugslikedoxorubicinindrug-sensitivetumorcells.thepurposeofthisstudyistoevaluatethedrugdeliverypotentialofaot–alginatenanoparticlesindrug-resistantcellsoverexpressingthedrugeffluxtransporter,p-glycoprotein(p-gp).aot–alginatenanoparticleswereformulatedusinganemulsion–cross-linkingprocess.rhodamine123anddoxorubicinwereusedasmodelp-gpsubstrates.cytotoxicityofnanoparticle-encapsulateddoxorubicinandkineticsofnanoparticle-mediatedcellulardrugdeliverywereevaluatedinbothdrug-sensitiveand-resistantcelllines.aot–alginatenanoparticlesenhancedthecytotoxicityofdoxorubicinsignificantlyindrug-resistantcells.theenhancementincytotoxicitywithnanoparticleswassustainedoveraperiodof10days.uptakestudieswithrhodamine-loadednanoparticlesindicatedthatnanoparticlessignificantlyincreasedthelevelofdrugaccumulationinresistantcellsatnanoparticledoseshigherthan200µg/ml.blanknanoparticlesalsoimprovedrhodamineaccumulationindrug-resistantcellsinadose-dependentmanner.nanoparticle-mediatedenhancementinrhodamineaccumulationwasnotbecauseofmembranepermeabilization.fluorescencemicroscopystudiesdemonstratedthatnanoparticle-encapsulateddoxorubicinwaspredominantlylocalizedintheperinuclearvesiclesandtoalesserextentinthenucleus,whereasfreedoxorubicinaccumulatedmainlyinperipheralendocyticvesicles.inhibitionofp-gp-mediatedrhodamineeffluxwithaot–alginatenanoparticleswasconfirmedinprimarybrainmicrovesselendothelialcells.inconclusion,anaot–alginatenanoparticlesystemenhancedthecellulardeliveryandtherapeuticefficacyofp-gpsubstratesinp-gp-overexpressingcells.
台式循环水真空泵使用方法
XY-MBR膜地埋式污水处理设备
医疗污水处理设备正确的管理方法
氢气发生器有哪些常见问题
2018年聚氨酯保温板价格及报价
90plus 纳米粒度仪应用案例-14
供应BY-CL1600便携式余氯、总氯检测仪（包邮）
我国突破航空发动机叶片超精细“冷加工”技术
防腐管道木托 防腐木块厂家
推动2023年数字乡村发展 强化农业科技和智能装备支撑
2019食检合格率达97.6% 检验仪器仪表助力品质监管
养殖用风机维修
防尘用绿网常卖尺寸
一体化污水处理设备的环保施工
必知必读1J87精密合金全面解析！
速度与激情！KSCAN为加足马力
玻璃棉毡的性质与用途
水面沙滩警戒围栏用途怎么样
解析不溶性微粒和可见异物的区别和检测方法
低温离心机的特点有哪些？