Anti-Phosphoserine/threonine抗体

anti-phosphoserine/threonine
from:abcam
catalog number :rs- 11994r
quantity size : 0.1ml (dilute with ph 7.4 0.01 m pbs or antibody diluent ) background: a hallmark of signal transduction pathways is the reversible phosphorylation of serine and threonine residues within specific sequences, or motifs, in target proteins. specific signaling motifs include not only sequences that are recognized by protein kinases, but also those that are recognized by phosphorylation-dependent binding proteins like 14-3-3. these modular phosphoprotein interacting domains are critical elements in modulating, directing and amplifying intracellular communications. many critical protein kinases can be regulated by phosphorylation at a specific serine or threonine surrounded by phenylalanine or tyrosine. for example, akt, an important kinase that regulates cell survival, is activated by phosphorylation at ser473, a site surrounded by phenylalanine and tyrosine. rsk1, p70 s6 k, and certain pkc isoforms also contain a similar consensus phosphorylation site. phosphorylation of these sites is required for kinase activity. the phospho-(ser/thr) phe antibody is a powerful tool for discovery of new proteins containing this important regulatory motif.
.specificity :anti-phosphoserine/threonine is a rabbit polyclonal antibody
specific for phosphoserine/threonine of phosphoserine/threonine
– use for western blotting,elisa,immunoprecipitation and immunohistochemistry
– protein g affinity chromatography purification, purity :>95%
– isotype: igg
application :
– wb=1:100-500elisa=1:500-1000ip=1:20-100ihc-p=1:100-500ihc-f=1:100-500if=1:100-500 klh conjugated synthetic peptide derived from ph phosphoserine/threonine
– not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user..
storage: store at -20℃ for one year. avoid repeated freeze/thaw cycles. the lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. when reconstituted in sterile ph 7.4 0.01m pbs or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
important note: this product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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