HPLC Pre-column and Post-column Derivatization Questions
1) why is derivatization performed? the purpose of derivatization is to convert analytes that cannot be directly detected into detectable substances by the detector. it allows the analytes to selectively react with derivatizing reagents, altering their elution order in the chromatographic column and achieving better separation.
2) what are the classifications of derivatization? based on the timing of derivatization, chemical derivatization can be classified into pre-column derivatization and post-column derivatization. pre-column derivatization involves converting the sample into derivative compounds before injection, followed by separation on the chromatographic column. post-column derivatization, on the other hand, involves modifying the physical or chemical properties of analytes after the chromatographic column to make them detectable.
3) what types of compounds are suitable for derivatization? derivatization is suitable for various compounds, including organic compounds, natural products, biopolymers (such as proteins, nucleic acids), environmental samples, food components, drugs, and drug metabolites, among others.
4) what are the requirements for derivatization? requirements for derivatization include selecting appropriate derivatizing reagents and reaction conditions to ensure selectivity and efficiency of the derivatization reaction. additionally, it is important to avoid the formation of multiple derivatization products that could complicate chromatographic separation.
5) what are the drawbacks of pre-column and post-column derivatization? the drawback of pre-column derivatization is the potential formation of multiple derivatization products, which can pose challenges in separation. furthermore, pre-column derivatization allows longer reaction times and various forms of reactors, but it requires additional processing steps and can be time-consuming. post-column derivatization, on the other hand, requires the development of specific post-column derivatization systems, involving criteria such as performance, price, and form. additionally, post-column derivatization may increase the complexity of the instrument and require adaptation of the chromatographic detector before and after the derivatization process.
LUGB-2308涡街流量计测量压缩空气时遇到故障解决方法
阴阳床再生污水pH控制
发泡橡塑保温管*
振动筛出现漏料怎么解决
中央空调垫木质量可靠厂家
HPLC Pre-column and Post-column Derivatization Questions
不间断回收闲置二手平板吊带离心机
投入式液位变送器在使用中常见的误差有哪些？
一小时60吨铝灰球磨机3个时代特性
cems烟气排放在线监测系统设备安装场所的选定
数字汽车衡的使用和维修方法
汽车衡泡水后的处理方法
复合三色彩条布生产价格
高温红外分析仪的分析原理和特点
雕刻机的冷却切削液使用注意事项
喷雾干燥机和闭式喷雾干燥系统的介绍
关于恒温光照培养箱的特点，你知道多少？
德阳难燃橡塑保温板价格查询
浅析卫生间污水提升器的工作原理
温度冲击试验箱的安装与调试技巧
2) what are the classifications of derivatization? based on the timing of derivatization, chemical derivatization can be classified into pre-column derivatization and post-column derivatization. pre-column derivatization involves converting the sample into derivative compounds before injection, followed by separation on the chromatographic column. post-column derivatization, on the other hand, involves modifying the physical or chemical properties of analytes after the chromatographic column to make them detectable.
3) what types of compounds are suitable for derivatization? derivatization is suitable for various compounds, including organic compounds, natural products, biopolymers (such as proteins, nucleic acids), environmental samples, food components, drugs, and drug metabolites, among others.
4) what are the requirements for derivatization? requirements for derivatization include selecting appropriate derivatizing reagents and reaction conditions to ensure selectivity and efficiency of the derivatization reaction. additionally, it is important to avoid the formation of multiple derivatization products that could complicate chromatographic separation.
5) what are the drawbacks of pre-column and post-column derivatization? the drawback of pre-column derivatization is the potential formation of multiple derivatization products, which can pose challenges in separation. furthermore, pre-column derivatization allows longer reaction times and various forms of reactors, but it requires additional processing steps and can be time-consuming. post-column derivatization, on the other hand, requires the development of specific post-column derivatization systems, involving criteria such as performance, price, and form. additionally, post-column derivatization may increase the complexity of the instrument and require adaptation of the chromatographic detector before and after the derivatization process.
LUGB-2308涡街流量计测量压缩空气时遇到故障解决方法
阴阳床再生污水pH控制
发泡橡塑保温管*
振动筛出现漏料怎么解决
中央空调垫木质量可靠厂家
HPLC Pre-column and Post-column Derivatization Questions
不间断回收闲置二手平板吊带离心机
投入式液位变送器在使用中常见的误差有哪些？
一小时60吨铝灰球磨机3个时代特性
cems烟气排放在线监测系统设备安装场所的选定
数字汽车衡的使用和维修方法
汽车衡泡水后的处理方法
复合三色彩条布生产价格
高温红外分析仪的分析原理和特点
雕刻机的冷却切削液使用注意事项
喷雾干燥机和闭式喷雾干燥系统的介绍
关于恒温光照培养箱的特点，你知道多少？
德阳难燃橡塑保温板价格查询
浅析卫生间污水提升器的工作原理
温度冲击试验箱的安装与调试技巧